New technique could advance reproductive science

A new study by researchers at Shinshu University holds promise for overcoming significant challenges in reproductive science, marking a major advancement in fertility research.

Mature egg cells, or oocytes, are essential for fertilization in assisted reproductive technologies.

However, some ‘denuded’ oocytes, or those lacking the protective granulosa cell layer, fail to mature.

Now, researchers at Shinshu University have developed a method to culture mature oocytes from these denuded oocytes in the lab.

The new approach holds promise for overcoming significant challenges in reproductive science, marking a major advancement in fertility research.

Assisted reproductive technology (ART) is a fertility treatment where eggs are removed from the ovaries, combined with sperm outside the human body, and the resulting embryos are placed in the uterus.

Typically, eggs or oocytes grow within fluid-filled sacs called follicles in the ovary.

Granulosa cells (GCs) surround the developing egg and provide essential nutrients and hormones.

However, early-stage follicles have only a thin GC layer, and during isolation for fertility treatments, these cells may detach from the egg, leaving behind “denuded” oocytes, which are unable to mature and cannot be used in ART.

Now, in a study published online on 21 August 2024 in Biology of Reproduction, Assistant Professor Kanako Morohaku and her team at Shinshu University have identified a novel method to produce viable, embryo-forming eggs from denuded oocytes taken from “preantral” follicles in mice.

Preantral follicles represent an earlier developmental stage, unlike the more mature antral follicles typically used in ART.

While previous research focused on antral follicles, there have been limited attempts to obtain viable eggs from preantral follicles.

To address this issue, the researchers isolated denuded oocytes and GCs from mice, which were then grown together in a U-shaped lab dish for eight days.

This led to the formation of granulosa cell-oocyte complexes, or GOCs, which allowed the oocytes to interact with supporting cells again.

In a previous study, the team found that oocyte quality was affected by the oxygen concentration under which GOCs were cultured.

Hence, these oocytes were cultured with GCs under high (20 per cent) and low (5 per cent) concentrations of oxygen to observe the difference in oocyte growth.

They observed that low oxygen levels significantly improved the development of GOCs and stimulated the formation of transzonal projections, thin cellular connections necessary for oocyte growth.

After eight days, these reassembled GOCs were cultured on a collagen-coated membrane for four additional days, leading to the maturation of oocytes into fertilizable eggs.

To test their viability, the researchers fertilized the eggs and transferred 126 embryos into female mice, following which they obtained six live pups.

Mr Tomohiro Koham is a PhD student and one of the authors.

He said: “To the best of our knowledge, our new culture protocol is the first to successfully obtain offspring from preantral follicle-derived denuded oocytes grown by reconstructing GOCs in vitro.”

The researchers suggest that this new method could also benefit large animals like cattle and pigs, where typical egg maturation techniques often fail due to the unique structure of their ovaries.

Additionally, the approach could help converse endangered species by supporting ex vivo (outside an organism’s body) production, offering a new way to preserve genetic resources.

Image: Kanako Morohaku at Shinshu University